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. 2018 Feb 15;7(1):1435138. doi: 10.1080/20013078.2018.1435138

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Detection of CSPG4+ exosomes immunocaptured on beads by flow cytometry. Exosomes are captured on streptavidin-labelled beads using pre-titered biotin-labelled anti-CSPG4 mAb (763.74 mAb). The antigen (CSPG4) carried by the bead-bound exosomes is detected using a fluorochrome-labelled and pre-tittered detection anrti-CSPG4 mAb (225.28 mAb). The flow cytometry-based detection provides the relative fluorescence intensity (RFI) value for exosomes carrying the antigen. RFI = MFI of detection Ab/MFI of isotype control Ab.