Figure 3.

Flow cytometry-based detection of CSPG4 antigen or CD63 antigen carried on exosomes which were immunocaptured with anti-CSPG4 mAb from plasma of five melanoma patients. Note that the RFI values for CSPG4+MTEX captured from plasma of these patients varied, and that the frequency of CSPG4+ exosomes corresponds to that of CD63+ exosomes, suggesting that most of captured MTEX are CD63 +. The asterisks (patients #6 and #8) indicate patients with the highest RFIs and highly advanced metastatic disease (see Supplementary Table 1).