Fig. 4.

Two-dimensional size-and-shape distribution analyses of the sedimentation velocity AUC data. a NPM1, b NPM1^N240, c NPM1^N188, and d NPM1^CTD. The transformed velocity data are shown as contour plots (heat maps) of c(M, f/f₀,) (top), c(s, f/f₀) (middle), and c(s, M) (bottom) with 0 fringes/S (white) to maximum value fringes/S (red), with increasing color temperature indicating higher values. Velocity data were acquired at 50,000 rpm at 20 °C in buffer comprised of 10 mM Tris, pH 7.5, 150 mM NaCl, 2 mM DTT. The s_w,(f/f₀)_w, and M-values are listed in Supplementary Table 2