Fig. 5.

Only NPM1 constructs with an intact IDR undergo homotypic LLPS. a Phase diagrams for NPM1^WT, NPM1^N240 and NPM1^N188 in buffer comprised of 10 mM Tris, 150 mM NaCl, 2 mM DTT, pH 7.5 in the presence of 150 mg/mL Ficoll PM70. The purple dotted line is a visual aid that represents the phase boundary for NPM1^WT between the mixed (gray circles) and demixed (green circles) states. Representative microscopy images are shown for 20 µM protein samples. Scale bar = 5 µm; b confocal microscopy images of homotypic NPM1 droplets at 20 µM NPM1 in the presence of 150 mg/mL Ficoll PM70, in buffers containing the indicated NaCl concentration. Scale bar = 10 µm