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. 2018 Feb 26;8:3620. doi: 10.1038/s41598-018-21866-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of different HSP inhibitors on in-vitro ovule culture at different stages of cotton fiber development. (A) Photographs of in-vitro cultured ovules in initiation, elongation and SCW stage showing the effect of Pif (HSP70) and Nov (HSP90) inhibitors. (B) SEM of control and treated ovules at 1 DPA shows clear decline in fiber growth. (C) Quantitative estimation of fiber growth in initiation, elongation and SCW stage at different concentration of Pif and Nov inhibitors (X-axis and Y-axis designates O.D. and concentration of inhibitors, respectively). The asterisks represent statistical significance between three biological and three technical replicates (*p-value ≤ 0.05, **p-value ≤ 0.01).