Figure 4.
Biochemical alterations due to HSP inhibitor treatment in in-vitro cultured ovules. (A) Relative H2O2 estimation in Nov and Pif treated ovules at 0 DPA. (B) The mean fluorescence at the end point of Nov and Pif treated ovules as compared to control ovules at 0 DPA. The asterisks represent statistical significance between three biological and two technical replicates (*p-value ≤ 0.05, **p-value ≤ 0.01). (C) Relative H2O2 estimation in Nov and Pif treated ovules at 6 DPA (D) The mean fluorescence at the end point of Nov and Pif treated ovules as compared to control ovules at 6 DPA. The asterisks represent statistical significance between three biological and two technical replicates (*p-value ≤ 0.05, **p-value ≤ 0.01). (E) Quantitative estimation of Ascorbate peroxidase (APX) activity in Nov and Pif treated ovules at 0 DPA (**p-value ≤ 0.01).