Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Feb 26;8:3615. doi: 10.1038/s41598-018-22053-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Differentiation into RAX+ hypothalamic progenitors. (A) Schematic of 3D culture (SFEBq) start conditions. (B) Failure of aggregation in SFEBq with gfCDM without KSR. (C) FOXG1 (white) expression in day 20 aggregates cultured with gfCDM containing KSR. FOXG1 is a telencephalic marker. (D) Induction of neural retina in day 30 aggregates in SFEBq with gfCDM + KSR and with BMP4 treatment. RAX::Venus (green), VSX2 (red). (E) Increased FOXG1 expression in day 20 aggregates treated with SAG in gfCDM + KSR. RAX::Venus (green), FOXG1 (red). (F) Culture protocol for induction of hypothalamic progenitors. (G–J) Immunostaining of day 30 aggregates with both BMP4 and SAG treatment in gfCDM + KSR. (G) RAX::Venus (green)+, VSX2 (red) −indicated hypothalamic progenitors. (H) Mixed pattern of dorsal (PAX6+) and ventral (NKX2.1+) hypothalamic precursors. RAX::Venus (green), PAX6 (red), NKX2.1 (white). (I,J) Expression of neural ectoderm markers. RAX::Venus (green), SOX1 (I, red) and NESTIN (J, red). (K) Time courses of RAX::Venus expression. Upper panels: bright field view, lower panels: fluorescence views of RAX::Venus+ cells. (L) FACS-analysis of day 20 aggregates. Over 80% of cells were induced to RAX+ hypothalamic progenitors. Green: BMP4 (+)/SAG (+), Purple: BMP4 (−)/SAG (−). For all relevant panels, nuclear counter staining: DAPI (blue), scale bar: 50 μM.