. 2018 Feb 26;8:3643. doi: 10.1038/s41598-018-22033-1

Table 2.

Activation and Steady-State Fast Inactivation.

Condition	GV-V_1/2 (mV)	GV-z	N	SSFI-V_1/2 (mV)	SSFI-z	N
WT - 22 °C - 0 nM Ca²⁺ - 0 µM Ran	−40.45 ± 1.24	3.76 ± 0.33	7	−88.62 ± 1.99	−3.93 ± 0.26	7
WT - 22 °C - 0 nM Ca²⁺ - 10 µM Ran	−45.59 ± 1.09	5.37 ± 0.29	6	−86.51 ± 1.36	−3.95 ± 0.16	7
WT - 22 °C - 0 nM Ca²⁺ - 100 µM Ran	−44.13 ± 3.07	4.84 ± 0.56	5	−88.45 ± 3.18	−2.87 ± 0.11^*2	6
WT - 22 °C - 2500 nM Ca²⁺ - 0 µM Ran	−42.22 ± 1.16	4.08 ± 0.25	9	−91.72 ± 1.72	−3.68 ± 0.15	9
WT - 22 °C - 2500 nM Ca²⁺ - 10 µM Ran	−44.40 ± 2.22	3.87 ± 0.28	7	−91.44 ± 1.63	−3.93 ± 0.18	7
WT - 22 °C - 2500 nM Ca²⁺ - 100 µM Ran	−44.76 ± 2.09	4.38 ± 0.36	9	−90.94 ± 1.64	−2.20 ± 0.13^*2	7
WT - 34 °C - 0 nM Ca²⁺ - 0 µM Ran	−43.44 ± 2.06	6.00 ± 0.45	6	−80.31 ± 1.17	−4.72 ± 0.18	6
WT - 34 °C - 0 nM Ca²⁺ - 10 µM Ran	−37.93 ± 1.97	4.95 ± 0.28	7	−82.51 ± 2.26	−4.00 ± 0.14	8
WT - 34 °C - 0 nM Ca²⁺ - 100 µM Ran	−37.58 ± 2.03	4.43 ± 0.33	7	−87.30 ± 2.73	−2.82 ± 0.16^*2	7
WT - 34 °C - 2500 nM Ca²⁺ - 0 µM Ran	−40.62 ± 1.44	5.96 ± 0.35	6	−80.25 ± 2.30	−4.24 ± 0.08	7
WT - 34 °C - 2500 nM Ca²⁺ - 10 µM Ran	−38.03 ± 3.69	5.15 ± 0.40	6	−83.18 ± 3.95	−4.00 ± 0.13	6
WT - 34 °C - 2500 nM Ca²⁺ - 100 µM Ran	−43.69 ± 0.98	6.30 ± 0.48	6	−90.43 ± 2.80	−3.18 ± 0.2^*2	6
EK - 22 °C - 0 nM Ca²⁺ - 0 µM Ran	−35.13 ± 2.70	2.99 ± 0.27	8	−100.46 ± 1.51	−2.97 ± 0.08	8
EK - 22 °C - 0 nM Ca²⁺ - 10 µM Ran	−35.83 ± 1.65	3.14 ± 0.27	10	−99.37 ± 1.61	−2.88 ± 0.08	10
EK - 22 °C - 0 nM Ca²⁺ - 100 µM Ran	−36.16 ± 1.87	3.95 ± 0.17	7	−103.83 ± 2.42	−1.77 ± 0.06^*2	8
EK - 22 °C - 2500 nM Ca²⁺ - 0 µM Ran	−39.33 ± 2.02	3.09 ± 0.16	5	−101.30 ± 3.14	−3.07 ± 0.21	6
EK - 22 °C - 2500 nM Ca²⁺ - 10 µM Ran	−34.88 ± 1.57	2.83 ± 0.15	8	−100.76 ± 1.83	−3.11 ± 0.13	8
EK - 22 °C - 2500 nM Ca²⁺ - 100 µM Ran	−33.72 ± 1.52	3.02 ± 0.35	5	−106.45 ± 2.29	−2.12 ± 0.08^*2	5
EK - 34 °C - 0 nM Ca²⁺ - 0 µM Ran	−30.22 ± 1.27	3.68 ± 0.28	9	−91.02 ± 2.79	−3.18 ± 0.14	9
EK - 34 °C - 0 nM Ca²⁺ - 10 µM Ran	−36.80 ± 1.20	4.31 ± 0.38	9	−94.20 ± 3.73	−3.14 ± 0.13	9
EK - 34 °C - 0 nM Ca²⁺ - 100 µM Ran	−35.53 ± 1.18	4.11 ± 0.22	8	−105.48 ± 1.64^*1	−2.07 ± 0.07^*2	8
EK - 34 °C - 2500 nM Ca²⁺ - 0 µM Ran	−34.74 ± 1.48	4.06 ± 0.41	8	−96.78 ± 4.70	−3.26 ± 0.12	8
EK - 34 °C - 2500 nM Ca²⁺ - 10 µM Ran	−31.25 ± 2.04	3.92 ± 0.35	7	−95.01 ± 3.23	−2.94 ± 0.15	7
EK - 34 °C - 2500 nM Ca²⁺ - 100 µM Ran	−32.33 ± 1.64	3.39 ± 0.15	9	−105.73 ± 2.44	−2.00 ± 0.06^*2	10

^*1p < 0.01 vs 0 µM and 10 µM Ran of same condition.

^*2p < 0.05 vs 0 µM and 10 µM Ran of same condition.