Table 3.
Open-State Fast Inactivation Time Constant.
| Condition | −50 mV τ (ms) | −30 mV τ (ms) | −10 mV τ (ms) | +10 mV τ (ms) | N |
|---|---|---|---|---|---|
| WT - 22 °C - 0 nM Ca2+ - 0 µM Ran | 3.47 ± 0.22 | 1.32 ± 0.11 | 0.69 ± 0.07 | 0.48 ± 0.04 | 7 |
| WT - 22 °C - 0 nM Ca2+ - 10 µM Ran | 3.28 ± 0.45 | 1.13 ± 0.06 | 0.61 ± 0.04 | 0.44 ± 0.03 | 5 |
| WT - 22 °C - 0 nM Ca2+ - 100 µM Ran | 5.72 ± 1.67*1 | 1.61 ± 0.20*2 | 0.74 ± 0.06 | 0.46 ± 0.03 | 5 |
| WT - 22 °C - 2500 nM Ca2+ - 0 µM Ran | 3.59 ± 0.49 | 1.34 ± 0.12 | 0.69 ± 0.03 | 0.47 ± 0.01 | 8 |
| WT - 22 °C - 2500 nM Ca2+ - 10 µM Ran | 3.03 ± 0.13 | 1.09 ± 0.05 | 0.58 ± 0.01 | 0.42 ± 0.01 | 6 |
| WT - 22 °C - 2500 nM Ca2+ - 100 µM Ran | 3.02 ± 0.28 | 1.30 ± 0.09 | 0.71 ± 0.05 | 0.49 ± 0.04 | 9 |
| WT - 34 °C - 0 nM Ca2+ - 0 µM Ran | 2.28 ± 0.52 | 0.66 ± 0.07 | 0.34 ± 0.02 | 0.23 ± 0.01 | 5 |
| WT - 34 °C - 0 nM Ca2+ - 10 µM Ran | 2.82 ± 0.46 | 0.70 ± 0.08 | 0.35 ± 0.04 | 0.23 ± 0.02 | 7 |
| WT - 34 °C - 0 nM Ca2+ - 100 µM Ran | 3.22 ± 0.45 | 0.78 ± 0.12 | 0.38 ± 0.06 | 0.22 ± 0.02 | 5 |
| WT - 34 °C - 2500 nM Ca2+ - 0 µM Ran | 2.43 ± 0.45 | 0.48 ± 0.04 | 0.27 ± 0.01 | 0.20 ± 0.01 | 6 |
| WT - 34 °C - 2500 nM Ca2+ - 10 µM Ran | 1.53 ± 0.24 | 0.60 ± 0.10 | 0.29 ± 0.03 | 0.20 ± 0.02 | 4 |
| WT - 34 °C - 2500 nM Ca2+ - 100 µM Ran | 2.36 ± 0.32 | 0.69 ± 0.08 | 0.38 ± 0.04 | 0.25 ± 0.02 | 6 |
| EK - 22 °C - 0 nM Ca2+ - 0 µM Ran | 1.35 ± 0.14 | 0.57 ± 0.03 | 0.40 ± 0.02 | 0.33 ± 0.02 | 7 |
| EK - 22 °C - 0 nM Ca2+ - 10 µM Ran | 1.46 ± 0.17 | 0.54 ± 0.03 | 0.36 ± 0.02 | 0.30 ± 0.02 | 10 |
| EK–22 °C - 0 nM Ca2+ - 100 µM Ran | 1.65 ± 0.16 | 0.61 ± 0.03 | 0.37 ± 0.01 | 0.29 ± 0.01 | 6 |
| EK - 22 °C - 2500 nM Ca2+ - 0 µM Ran | 0.71 ± 0.09 | 0.44 ± 0.02 | 0.32 ± 0.01 | 0.26 ± 0.01 | 6 |
| EK - 22 °C - 2500 nM Ca2+ - 10 µM Ran | 1.23 ± 0.09 | 0.54 ± 0.03 | 0.37 ± 0.01 | 0.30 ± 0.01 | 7 |
| EK - 22 °C - 2500 nM Ca2+ - 100 µM Ran | 1.65 ± 0.13 | 0.81 ± 0.09 | 0.54 ± 0.06*3 | 0.40 ± 0.03*4 | 5 |
| EK - 34 °C - 0 nM Ca2+ - 0 µM Ran | 0.89 ± 0.12 | 0.34 ± 0.02 | 0.23 ± 0.02 | 0.18 ± 0.01 | 9 |
| EK - 34 °C - 0 nM Ca2+ - 10 µM Ran | 0.88 ± 0.11 | 0.30 ± 0.02 | 0.21 ± 0.01 | 0.17 ± 0.01 | 9 |
| EK - 34 °C - 0 nM Ca2+ - 100 µM Ran | 1.41 ± 0.34 | 0.47 ± 0.09 | 0.34 ± 0.07 | 0.24 ± 0.05 | 8 |
| EK - 34 °C - 2500 nM Ca2+ - 0 µM Ran | 0.62 ± 0.11 | 0.27 ± 0.02 | 0.18 ± 0.01 | 0.14 ± 0.01 | 9 |
| EK - 34 °C - 2500 nM Ca2+ - 10 µM Ran | 1.08 ± 0.07 | 0.35 ± 0.05 | 0.22 ± 0.02 | 0.17 ± 0.01 | 6 |
| EK - 34 °C - 2500 nM Ca2+ - 100 µM Ran | 0.88 ± 0.11 | 0.37 ± 0.02 | 0.21 ± 0.01 | 0.16 ± 0.01 | 8 |
*1p < 0.05 vs 0 µM and 10 µM Ran of same condition.
*2p < 0.05 vs 10 µM Ran of same condition.
*3p < 0.01 vs 0 µM Ran of same condition.
*4p < 0 ± 0.05 vs 0 µM Ran of same condition.