. 2018 Feb 26;8:3643. doi: 10.1038/s41598-018-22033-1

Table 5.

Use-Dependence (1 Hz and 3 Hz).

Condition	1 Hz–y0	1 Hz–τ1 (s)	1 Hz–τ2 (s)	N	3 Hz–y0	3 Hz–τ1 (s)	3 Hz–τ2 (s)	N
WT - 22 °C - 0 nM Ca²⁺ - 0 µM Ran	0.84 ± 0.03	3.13 ± 0.88	47.88 ± 9.44	6	0.63 ± 0.04	2.03 ± 0.41	40.53 ± 5.42	7
WT - 22 °C - 0 nM Ca²⁺ - 10 µM Ran	0.77 ± 0.01	2.96 ± 0.52	18.55 ± 4.50	7	0.52 ± 0.02	3.39 ± 0.62	23.00 ± 7.54	7
WT - 22 °C - 0 nM Ca²⁺ - 100 µM Ran	0.44 ± 0.02^*1	2.19 ± 0.44	9.70 ± 3.31	5	0.15 ± 0.02^*1	0.92 ± 0.18	7.02 ± 4.44	4
WT - 22 °C - 2500 nM Ca²⁺ - 0 µM Ran	0.81 ± 0.02	8.22 ± 2.84	114.32 ± 22.42	7	0.66 ± 0.03	1.83 ± 0.23	36.4 ± 7.98	7
WT - 22 °C - 2500 nM Ca²⁺ - 10 µM Ran	0.78 ± 0.01	6.93 ± 0.94	31.93 ± 10.85	6	0.52 ± 0.02	1.19 ± 0.19	8.57 ± 0.42	5
WT - 22 °C - 2500 nM Ca²⁺ - 100 µM Ran	0.35 ± 0.03^*1	1.67 ± 0.12	34.32 ± 22.87	7	0.20 ± 0.03^*1	0.69 ± 0.32	14.56 ± 13.09	3
WT - 34 °C - 0 nM Ca²⁺ - 0 µM Ran	0.92 ± 0.01	4.77 ± 3.14	39.42 ± 20.36	4	0.84 ± 0.03	2.75 ± 1.00	17.73 ± 9.63	5
WT - 34 °C - 0 nM Ca²⁺ - 10 µM Ran	0.76 ± 0.05	5.41 ± 3.35	62.60 ± 20.25	5	0.65 ± 0.06	0.98 ± 0.10	16.65 ± 6.29	6
WT - 34 °C - 0 nM Ca²⁺ - 100 µM Ran	0.60 ± 0.06^*2	0.66 ± 0.10	43.51 ± 18.93	8	0.33 ± 0.05^*1	0.39 ± 0.08	13.99 ± 8.03	8
WT - 34 °C - 2500 nM Ca²⁺ - 0 µM Ran	0.88 ± 0.03	30.10 ± 6.41^*3	59.96 ± 37.08	4	0.79 ± 0.04	1.46 ± 0.17	38.85 ± 12.19	8
WT - 34 °C - 2500 nM Ca²⁺ - 10 µM Ran	0.82 ± 0.04	2.83 ± 1.02	67.05 ± 23.05	5	0.60 ± 0.03^*4	1.02 ± 0.16	17.28 ± 6.89	7
WT - 34 °C - 2500 nM Ca²⁺ - 100 µM Ran	0.64 ± 0.02	0.44 ± 0.16	8.44 ± 2.77	5	0.27 ± 0.03^*1	0.41 ± 0.06	17.80 ± 9.98	5
EK - 22 °C - 0 nM Ca²⁺ - 0 µM Ran	0.69 ± 0.06	3.37 ± 0.64	69.20 ± 25.09	6	0.79 ± 0.01	3.08 ± 1.61	32.65 ± 20.62	4
EK - 22 °C - 0 nM Ca²⁺ - 10 µM Ran	0.57 ± 0.05	3.25 ± 0.63	64.71 ± 37.46	7	0.47 ± 0.02^*4	2.40 ± 0.37	16.28 ± 3.57	6
EK - 22 °C - 0 nM Ca²⁺ - 100 µM Ran	0.27 ± 0.05^*1	1.87 ± 0.25	74.43 ± 28.56	8	0.14 ± 0.03^*1	1.00 ± 0.34	1.29 ± 0.19	4
EK - 22 °C - 2500 nM Ca²⁺ - 0 µM Ran	0.73 ± 0.02	1.99 ± 1.02	31.37 ± 6.74	4	0.69 ± 0.06	3.59 ± 1.84	8.20 ± 3.73	4
EK - 22 °C - 2500 nM Ca²⁺ - 10 µM Ran	0.59 ± 0.06	7.17 ± 1.35	75.52 ± 20.26	5	0.47 ± 0.04	3.38 ± 0.43	13.21 ± 1.51	5
EK - 22 °C - 2500 nM Ca²⁺ - 100 µM Ran	0.24 ± 0.04^*1	2.68 ± 1.13	70.86 ± 29.29	3	0.20 ± 0.06^*2	0.82 ± 0.29	1.94 ± 0.31	3
EK - 34 °C - 0 nM Ca²⁺ - 0 µM Ran	0.82 ± 0.04	6.76 ± 1.43	68.66 ± 25.39	8	0.77 ± 0.03	2.66 ± 0.45	49.11 ± 13.27	9
EK - 34 °C - 0 nM Ca²⁺ - 10 µM Ran	0.77 ± 0.06	2.40 ± 0.55	78.22 ± 35.32	7	0.55 ± 0.03^*4	1.63 ± 0.32	27.16 ± 15.43	5
EK - 34 °C - 0 nM Ca²⁺ - 100 µM Ran	0.47 ± 0.04^*1	0.77 ± 0.07	19.07 ± 9.49	6	0.21 ± 0.05^*1	0.40 ± 0.04	24.08 ± 18.78	5
EK - 34 °C - 2500 nM Ca²⁺ - 0 µM Ran	0.87 ± 0.03	15.05 ± 3.35^*3	65.60 ± 19.19	5	0.74 ± 0.08	2.50 ± 0.65	30.82 ± 2.43	6
EK - 34 °C - 2500 nM Ca²⁺ - 10 µM Ran	0.77 ± 0.04	1.77 ± 0.48	48.78 ± 22.76	5	0.63 ± 0.03	1.13 ± 0.12	6.41 ± 3.13	6
EK - 34 °C - 2500 nM Ca²⁺ - 100 µM Ran	0.46 ± 0.05^*1	1.21 ± 0.20	38.24 ± 19.24	5	0.26 ± 0.03^*1	0.54 ± 0.14	6.89 ± 4.62	5

^*1p < 0.0001 vs 0 µM and 10 µM Ran of same condition.

^*2p < 0.0001 vs 0 µM Ran of same condition.

^*3p < 0.0001 vs 10 µM and 100 µM Ran of same condition.

^*4p < 0.0001 vs 0 µM and 100 µM Ran of same condition.