Figure 8. .

(A) siNoggin release behavior from 22.5% (w/v) photodegradable hydrogels into DMEM-LG in the absence or presence of ‘UV 2–10’ daily. (B) Photomicrographs of ARS staining depicting the deposition of calcium, and (C) quantification of deposited calcium (normalized to ‘Ctrl’) by hMSCs cultured in monolayer and transfected with the same concentration (40 nM) of released siNoggin from hydrogels at day 2 (D2) or day 4 (D4) or freshly reconstituted lyophilized siRNA/PEI complexes of siCT (‘siCT’) or siNoggin (‘Pos. Ctrl’). ‘Gel only’: pooled D4 releasates from ‘UV’-treated hydrogels lacking RNA. The scale bar indicates 200 μm.

^#p < 0.05 compared with groups that do not contain the same symbol.

*p < 0.05.