Table 1. PCR Primers for Amplification of cagA and vacA Genes.
Region | Primer | Primer sequence | PCR product size (bp) |
---|---|---|---|
cagA | F1 | GAGCAATCGCTTACGCTCAG | 250 |
R1 | GTGAATGGAACCCTAGTCGG | ||
vacA | |||
m1 | VA3-F | GGTCAAAATGCGGTCATGG | 290 |
VA3-R | CCATTGGTACCTGTAGAAAC | ||
m2 | VA4-F | GGAGCCCCAGGAAACATTG | 352 |
VA4-R | CATAACTAGCGCCTTGCAC | ||
s1b | SS3-R | AGCGCCATACCGCAAGAG | 187 |
VA1-R | CTGCTTGAATGCGCCAAAC | ||
s1a | SS1-R | GTCAGCATCACACCGCAAC | 190 |
VA1-R | CTGCTTGAATGCGCCAAAC | ||
s2 | SS2-R | GCTAACACGCCAAATGATGC | 199 |
VA1-R | CTGCTTGAATGCGCCAAAC |