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. Author manuscript; available in PMC: 2018 Nov 13.
Published in final edited form as: Cancer Cell. 2017 Nov 13;32(5):654–668.e5. doi: 10.1016/j.ccell.2017.10.005

Figure 4. Effect of CSF1 on fibroblasts’ ability to recruit PMN-MDSC.

Figure 4

A. Expression of CD115 on the surface of fibroblasts tested by FACs and in cell lysates of cultured CAF tested by western blot B. Expression of Cxcl1 mRNA (qPCR) (left) and Cxcl-1 protein (ELISA) (right) in normal lung fibroblasts treated with Csf1 with and without JNJ-40346527. Mean and SD are shown (n=3). **- p<0.01, *** - p<0.001 in Student’s t-test from control group. C. Effect of Csf1 on fibroblast-mediated recruitment of BM PMN-MDSC. Chemotaxis of BM PMN-MDSC from LLC-TB mice was evaluated against supernatants obtained from fibroblasts cultured with Csf1 and Csf1R inhibitor. *- p<0.05 in Student’s t-test from control group (n=4). D. PMN-MDSC chemotaxis to supernatants from fibroblasts in presence of neutralizing Csf1 antibody (Ab). *- p<0.05 from control group in Student’s t-test (n=3). E. PMN-MDSC chemotaxis to supernatants from fibroblasts in presence of neutralizing CXCL1 Ab. **-p<0.01 from control group in Student’s t-test. F. PMN-MDSC chemotaxis to recombinant CXCL1 (100 ng/ml) in the presence of Csf1 and CSF1R inhibitor ***- p<0.001 in Student’s t-test from control group (n=3). G. Csf1 ELISA with TES collected from control or JNJ-40346527-treated LLC TB mice. *- p<0.05 in Student’s t-test from control group (n=4). H. PMN-MDSC chemotaxis in presence of TES collected from control or JNJ-40346527-treated LLC TB mice. *- p<0.05 in Student’s t-test from control group (n=4). I. LLC tumor growth kinetics in WT and CXCR2 KO mice with or without JNJ-40346527 treatment for indicated days. J. The absolute counts of PMN-MDSC and TAM in tumors of WT or CXCR2 KO TB mice treated with JNJ-40346527. *- p<0.05 in Student’s t-test from control group (n=4). See also Figure S4.