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. Author manuscript; available in PMC: 2018 Feb 27.
Published in final edited form as: Sci Signal. 2017 Dec 12;10(509):eaan6282. doi: 10.1126/scisignal.aan6282

Figure 3. CRP enhances myeloma-induced OC formation and activity in vitro.

Figure 3

(A and B) The number of multinuclear TRAP+ cells (A) and the amount of secreted TRAP5b (B) in cultures of OC precursor cells cultured alone or with either primary myeloma cells isolated from five myeloma patients (Pt MM) or with human myeloma cell lines (MM) in medium without RANKL in the presence of CRP (5 μg/mL) or PBS. (C) Real-time PCR analysis of the expression of CALCR, CTSK, and TRAP in mature OCs cocultured with OC precursors and ARP-1 or MM.1S cells with or without CRP (5 μg/mL). (D) TRAP staining for the number of multinuclear TRAP+ cells in cocultures of OC precursors with vector- or CRP-expressing ARP-1 or MM.1S cells (MM-Vector or MM-CRP) without RANKL. (E) As in (D), in the presence of antibodies against CRP (αCRP; 5 μg/mL) or control IgG. *P < 0.05, **P < 0.01 by student’s t test. Data from four independent experiments are shown.