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. Author manuscript; available in PMC: 2018 Feb 27.
Published in final edited form as: Sci Signal. 2017 Dec 12;10(509):eaan6282. doi: 10.1126/scisignal.aan6282

Figure 5. Through myeloma cell CD32, CRP enhances the production of cytokines that mediate OC formation and activation.

Figure 5

(A to D) Real-time PCR analysis of MCP-1, MIP-1α, and RANKL mRNA expression (A, B) and ELISA analysis of secreted protein (C, D) in CD32-knockdown (CD32-KD) ARP-1 or MM.1S cells cultured with CRP (5 μg/mL), compared with those in non-target control shRNA-transfected (Ctrl) cells. (E to H) Analysis of cytokine (MCP-1, MIP-1α, and RANKL) abundance at the mRNA (E, F) and protein (G, H) levels in cultures of vector- or CD32-transfected (CD32) ARP-1 or MM.1S cells cultured with or without CRP (5 μg/mL). *P < 0.05, ** P < 0.01 by student’s t test. Data from three independent experiments are shown.