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. Author manuscript; available in PMC: 2018 Aug 30.
Published in final edited form as: J Am Chem Soc. 2017 Aug 15;139(34):11964–11972. doi: 10.1021/jacs.7b06152

Figure 2.

Figure 2

Split RNAP biosensors can detect Bcl-2 family PPIs in mammalian cells. (A) Vector system to test binary Bcl-2 split RNAP PPI detection system in mammalian cells using PPI driven GFP mRNA as the RNA output. (B) HEK293T cells cotransfected with the plasmids shown in (A). 30 h after transfection, the cells were analyzed for GFP expression by fluorescence microscopy. Interactions between tBID and Bcl-2, Mcl-1, Bcl-XL, and Bcl-W, are readily detected compared to control, while NOXA is much more selective for interaction with Mcl-1. (C) Quantification of (B) (error bars are ± s.e.m, n = 5). Student’s t-test; **P < 0.001. 100 μm scale bar shown.