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. Author manuscript; available in PMC: 2018 Feb 27.
Published in final edited form as: Cell Rep. 2018 Jan 30;22(5):1211–1224. doi: 10.1016/j.celrep.2018.01.017

Figure 3. Transposon Insertions in the Pi4ka Gene Are Associated with Blast-like Phenotype and Decreased Red Blood Cells.

Figure 3

(A) Transposon insertions throughout the Pi4ka gene.

(B) Pi4ka RNA sequencing counts per million for affected and C spleens.

(C) Pi4ka expression by qPCR of the individual spleens from affected mice compared to Cre− spleens (n = 6).

(D) H&E of Cre− spleens and those with Pi4ka insertions (scale bar, 600 mm). Pi4ka immunohisto-chemistry (IHC) in spleens of C and affected mice (top: scale bar, 600 μm; middle: scale bar, 60 μm). Bottom: Cytospins of RBC-lysed blood from C and affected animals (scale bar, 15 μm)

(E) CBC analysis in C and Pi4ka-affected animals. For Cs (Cre−), data are represented as mean ± SEM of n = 10 animals.