Fig. 5.

Chromosome preparation in the freshwater snail P. canaliculata. The optimized chromosome preparation protocol in planarians was used to generate karyotypes for both adult and embryonic tissues of the freshwater snail P. canaliculata. a–c Mitotic cells showing (a) early prophase, (b) late prophase or metaphase, and (c) anaphase in embryos 5 dpf without colchicine treatment. d Karyotype and e karyogram obtained from P. canaliculata embryos 5 dpf treated with colchicine. They are also representative of chromosome spreads obtained from the anterior and posterior kidney and the digestive gland in adult snails