Fig. 6.

Glycerol concentrations resulted in different protective effects on either sperm membrane integrity or motility-related characteristics. a Collagenase-treated sperm cryopreserved with 7.8% glycerol showed higher abnormality rate and lower viability when compared with 2.5% glycerol cryopreserved group. Differences were also observed on acrosome integrity that 7.8% glycerol group contained significantly lower acrosome intact spermatozoa after cryopreservation processes (72% vs. 51% for 2.5% and 7.8% glycerol group). b Collagenase-treated chimpanzee sperm cryopreserved with 7.8% glycerol exhibited higher motility, progressive motility, beat frequency cross (BCF) and linearity (LIN) when compared with 2.5% glycerol group; N.S: not significantly different. At least 200 sperm cells were evaluated in each sample. *Indicates significant differences between groups. Relative changes within each group (t60 vs. t10) were also indicated. Data were analyzed from 16 ejaculates from 4 individuals