Table 5.
Biochemical parameters in lean Zucker rats after repeated exposure to E171 and E153 by oral gavage
| In vivo Study | Control (n = 9) | E171 (n = 9) | E171 (n = 10) | E153 (n = 10) | E153 (n = 8) |
|---|---|---|---|---|---|
| Dose (mg/kg bw/week) | 0 | 50 | 500 | 0.64 | 6.4 |
| Vitamin C (μM) (ascorbate + DHA) | 19.6 ± 2.9 | 20.3 ± 2.6 | 21.0 ± 4.6 | 20.5 ± 3.9 | 18.9 ± 2.9 |
| MDA (μM) | 0.20 ± 0.05 | 0.19 ± 0.08 | 0.22 ± 0.07 | 0.20 ± 0.07 | 0.19 ± 0.05 |
| BH4 (nM) | 150.1 ± 20.4 | 149.2 ± 18.9 | 154.8 ± 43.7 | 151.5 ± 20.5 | 134.5 ± 27.4 |
| BH4/BH2 ratio (nM) | 0.20 ± 0.04 | 0.18 ± 0.04 | 0.19 ± 0.04 | 0.17 ± 0.05 | 0.21 ± 0.05 |
| ADMA (μM) | 178.1 ± 31.8 | 192.3 ± 41.0 | 179.1 ± 47.0 | 219.8 ± 118.1 | 215.1 ± 89.4 |
| L-arginine (μM) | 51.0 ± 8.8 | 61.8 ± 15.8 | 58.3 ± 16.9 | 75.5 ± 54.1 | 75.1 ± 35.5 |
All biochemical parameters were measured in plasma samples taken at the time of sacrifice. bw: body weight, DHA dehydroascorbic acid, MDA malondialdehyde, BH4 tetrahydrobiopterin, BH2 dihydrobiopterin, ADMA asymmetric dimethylarginine. Data are represented as mean ± SD