Table 1.

Primer sequences for control and target genes and q-PCR conditions

Symbol	Primer sequence	Amplification conditions	PCR product size (bp)	Accession number	Reference
TSG-6	S: GATGGATGGCTAAGGGCAGAGT-3′	93 °C, 20 s, 61 °C, 20 s 72 °C 30 s	208	NM_007115.3	Liu et al. 2016 [44]
	AS: TCATTTGGGAAGCCTGGAGATT-3′
EP2	S: 5-CCACCTCATTCTCCTGGCTA-3	93 °C, 20 s, 62 °C, 20 s 72 °C 30 s	216	NM_000956.3	Kunisch et al. 2009 [45]
	AS: 5-CGACAACAGAGGACTGAACG-3
HGF	S: 5′-CTCACACCCGCTGGGAGTAC-3′	93 °C, 20 s, 62 °C, 20 s 72 °C 30 s	104	XM_011516115.2	Jankowski et al. 2003 [46]
	AS: 5′-TCCTTGACCTTGGATGCATTC-3′
RUNX2	S: GCCTTCAAGGTGGTAGCCC-3′	93 °C, 20 s, 62 °C, 20 s 72 °C 30 s	67	XM_017011396.1	Shafiee et al. 2011 [47]
	AS: CGTTACCCGCCATGACAGTA-3′
OPN	S: 5′-TTGCAGCCTTCTCAGCCA-3′	93 °C, 20 s, 62 °C, 20 s 72 °C 30 s	76	NM_001251830.1	Bahrambeigi et al. 2012 [48]
	AS: 5′-GGAGGCAAAAGCAAATCACTG-3′
FABP4	S: 5′-ATGGGATGGAAAATCAACCA-3′	93 °C, 20 s, 59 °C, 20 s 72 °C 30 s	87	NM_001442.2	Ponnaiyan et al. 2014 [49]
	AS: 5′-GTGGAAGTGACGCCTTTCAT-3′
GAPDH	S: 5′-GACTCATGACCACAGTCCATGC-3′	93 °C, 20 s, 67 °C, 20 s 72 °C 30 s	112	NM_001289746.1	Liang et al. 2015 [50]
	AS: 5′-AGAGGCAGGGATGATGTTCTG-3′
NANOG	S: 5′-CAAAGGCAAACAACCCACT-3′	93 °C, 30 s, 60 °C, 30 s 72 °C 30 s	427	NM_024865.2	Kozhukharova et al., 2009 [23]
	AS: 5′-CTGGATGTTCTGGGTCTGGT-3′
OCT4	S:5′-AGCCCTCATTTCACCAGGCC-3′	93 °C, 30 s, 63 °C, 30 s 72 °C 30 s	456	NM_002701.5	Liedtke et al., 2007 [51]
	AS:5′-TGGGACTCCTCCGGGTTTTG-3′
SOX2	S:5′ GCGCATGGACAGTTACGC-3′	93 °C, 30 s, 60 °C, 30 s 72 °C 30 s	276	NM_003106.3	Koshkin et al. 2016 [52]
	AS: 5′ TCGGACTTGACCACCGAAC-3′
ACTIN	S:5′-GCCGAGCGGGAAATCGTGCGT-3′	93 °C, 30 s, 70 °C, 30 s 72 °C 30 s	507	NM_001101.3	Alekseenko et al., 2012 [53]
	AS:-5’-CGGTGGACGATGGAGGGGCCG-3′