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. 2017 Dec 20;9(12):10294–10306. doi: 10.18632/oncotarget.23516

Figure 3. p35 has profibrotic effects in mesenchymal cells.

Figure 3

(A) Real-time qPCR. Mouse 3T3-L1 preadipocytes were incubated with differentiation media for 2 days followed by maintenance media for 3 days, transfected with p35 and incubated with TGF-β (10 ng/ml) for a further 48 h. Results, normalized to 36B4, represent means ± SD of triplicate determinations from an experiment representative of three. *p < 0.05. (B, C) Foreskin fibroblasts were infected with Ad-p35 (30 MOI) and incubated with TGF-β for 72 h. (B) Western analysis. Representative images. (C) Real-time qPCR results, normalized to GAPDH, represent means ± SD of triplicate determinations from an experiment representative of three. *p < 0.05.