Figure 5. Inhibition of CDK5 abrogates TGF-ß responses in skin organ cultures.

Skin explants were incubated with TGF-β (10 ng/ml) for 6 d, with roscovitine (10 μM) added to the media 30 min before (pre-treatment) or 48 h following (post-treatment) TGF-β. (A) Real-time qPCR results, normalized with GAPDH, are means ± SD of triplicate determines from a representative experiment. ^*p < 0.05. (B) Western analysis. Representative images.