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SSc skin fibroblasts (n = 6) were incubated with roscovitine (10 μM) for 48 h. (A) Western analysis of whole cell lysates and culture supernatants. Left panels, Representative images. Cgn I, Type I collagen. Right panel, band intensities normalized with GAPDH levels expressed relative to untreated cultures. (B) Real-time qPCR. Results are expressed as dot plots of mRNA levels relative to untreated cultures.
