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Proliferation of NME overexpressing MDA-MB-231T A. and MDA-MB-435 cells B. after 24 and 48 hrs was assessed using AlamarBlue dye. The NDPK activity of NME overexpressing MDA-MB-231T C. and MDA-MB-435 D. cell lysates was quantified by a spectrophotometric assay for the formation of NAD (oxidized) from NADH (reduced) leading to the loss of OD at 340 nm. All experiments are representative of 4 replicates conducted.
