Figure 2. NDRG1-OT1_v4 is up-regulated in hypoxia and down-regulated in re-oxygenation.

(A) Validation of lnc-CPN2-1, lnc-C11orf35-2, lnc-NDRG1-1 and MALAT1 expression levels among different O₂ conditions by quantitative RT-PCR. Internal control: 18s. Positive control for hypoxia: MALAT1 [33]. The relative expression level of each condition was normalized to O₂. Data were repeated at least 3 times, and the results are means ± SDs. ^*, P <0.05. (B) Schematic diagram of NDRG1-OT1 isoforms. Transcription of NDRG1-OT1 was overlapped with NDRG1, located in chromosome 8. The exon number and position are shown on the sequence of NDRG1. v1-v5 indicate the different isoforms. (C) Expression profiling of NDRG1-OT1_v4 in six breast cancer cells and one breast normal cell line (MCF-10A) under different O₂ conditions.