Fig. 5.

Baf200 is required for adult hematopoiesis in steady state. a Representative FACS profiles showing hematopoietic stem and progenitor cells (HSPCs) in the BM from WT and Vav-iCre⁺, Baf200^f/f mice. b Graph showing the relative frequency (top) and absolute cell number (bottom) of the indicated subsets in the BM from WT and Vav-iCre⁺, Baf200^f/f mice (n = 5–9 per genotype for each subset). c Representative FACS profiles showing hematopoietic progenitors in the spleen from male WT and Vav-iCre⁺, Baf200^f/f mice (8–10-week-old). d Graph showing the percentage (left) and absolute cell number (right) of the indicated subsets in the spleen from male WT and Vav-iCre⁺, Baf200^f/f mice (8–10-week-old) (n = 6 per genotype for each subset). e Scatter plots of all expressed genes in BM LSK cells from WT and Vav-iCre⁺, Baf200^f/f mice. Blue indicates downregulation gene, yellow indicates upregulation gene, and grey indicates non-regulation gene in Vav-iCre⁺, Baf200^f/f LSK cells. f Upregulation genes and downregulation genes in Vav-iCre⁺, Baf200^f/f LSK cells were confirmed by RT-qPCR (n = 2 per genotype). Data are shown as means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001