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. 2018 Feb 27;18:17. doi: 10.1186/s12866-018-1160-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Phylogenetic tree of the FCT₁-region and its relation to phenotypic binding abilities. The phenotypic binding abilities of the SDSE isolates concorded strongly with genetic variations in the FCT₁-region. Based on their affinity for fibronectin, collagen and stainless steel, the isolates could be segregated into four distinct categories. Isolates highlighted in green adhered strongly to fibronectin and collagen, whereas isolates highlighted in yellow bound poorly to both substances. The blue marking signifies high-level binding to fibronectin and a low affinity for collagen. Inversely, the isolates highlighted in the red adhered well to collagen, but not to fibronectin. The isolates highlighted in yellow had a strong affinity for stainless steel, whereas the other isolates were characterized by a medium binding ability to stainless steel. Isolates marked with an asterisk had phenotypic binding characteristics deviating from their colour-coded category. Four isolates were phylogenetic outliers. Scale indicates substitutions per site. The phylogenetic tree was constructed in Geneious by global alignment with free end gaps using the Jukes-Cantor genetic distance model [38]. FastTree was used for visualising and tree-rendering [39]