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. 2017 Dec 20;27:225–236. doi: 10.1016/j.ebiom.2017.12.014

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — Knocking-down DLL4 affects sprouting angiogenesis and microvessel integrity.

(a) Phase-contrast images of microvessels made of HUVEC transfected with siControl (siCtl) or siDLL4 and treated with 50 ng/mL VEGF-A for 10 days (green arrows: sprouts, red arrows: migrating cells). (b & c) Actin and VE-cadherin (VE-Cad) immunostaining on microvessels. (b) Maximum intensity projection of LSFM 3D images showing the general morphology of microvessels (*Indicates another part of microvessel seen in background due to the LSFM method). (c) Maximum intensity projection of CLSM images showing details of the adherens-junctions and actin cytoskeleton (arrows: actin stress fibers).