Fig. 5.

Analysis of angiogenic sprouts using optical coherence tomography.

(a) 3D reconstructed images of microvessels made of HUVEC transfected with siControl (siCtl) or siDLL4 and treated with 50 ng/mL VEGF-A for 10 days (lumen and side views). (b) Example of computational modeling of an angiogenic sprout of interest for measurement of its dimensions (white arrows: selected sprout). (c) Average length, volume, surface area and diameter measured from 3D binary images of angiogenic sprouts in a 2-mm-long area of microvessels treated with VEGF-A for 10 days (n = 10 sprouts, error bars: S.D.).