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. 2017 Dec 13;27:258–274. doi: 10.1016/j.ebiom.2017.12.011

Fig. 5.

Fig. 5

HtrA1 overexpression leads to apical impairment. (A) IF confocal images of RPE at day 35 of culture. β-tubulin (red) and DAPI (blue). Left image, S328A overexpressing cells; right image, HtrA1 overexpressing cells. Scale bar, 50 μm. Close up images show ciliated cells. (A′) % of ciliated cells in the RPE population. Data represent the mean ± SD of three independent experiments, ***p < 0.001. See also Fig. S3. (B) Life cell imaging of RPE cells at day 35 of culture. Left, S328A overexpressing RPE; right, HtrA1 overexpressing RPE. The apical membrane faces the top. Scale bar, 10 μm. (C) Transmission Electron micrographs of S328A (above) and Htra1 (below) overexpressing cells. Scale bar, 5 μm. (C′) Microvilli per μm of apical membrane. Data represent the mean ± SD of one experiment, ***p < 0.001.