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. 2018 Jan 1;32(1):79–92. doi: 10.1101/gad.309245.117

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© 2018 Pontes and Groisman; Published by Cold Spring Harbor Laboratory Press

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Figure 3. — The Mg²⁺ transporters MgtA and MgtB and the ATPase inhibitor MgtC regulate PhoB activity in opposite ways. (A) Fluorescence from wild-type Salmonella (14028s) harboring pPhoB-GFP (phoB-gfp), pMgtA-leader-GFP (mgtA-gfp), pMgtC-leader-GFP (mgtC-gfp), or the promoterless GFP vector pVector plasmid. (B) Fluorescence from wild-type (14028s), mgtA mgtB (EG17048), and mgtC (EL4) Salmonella harboring pPhoB-GFP or pVector. (C) Fluorescence from wild-type (14028s), mgtA mgtB (EG17048), mgtC (EL4), and mgtA mgtCB (MP363) Salmonella harboring pPstS-GFP or pVector. (D) Fluorescence from wild-type (14028s), phoB (EG9054), mgtA mgtB (EG17048), and mgtA mgtB (EG17048) and phoB mgtA mgtB (MP1184) Salmonella following 24 h of growth on solid medium. (E) Fluorescence from wild-type Salmonella (14028s) harboring pPhoB-GFP_AAV, pPstS-GFP_AAV, or pVector_AAV. For all experiments, cells were grown in MOPS liquid or on solid (1% agarose) medium containing 10 µM MgCl₂ and 500 µM K₂HPO₄. Error bars represent the standard deviations. Graphs are representative of at least three independent experiments with a total of at least six biological replicates. See also Supplemental Figure S2.