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. 2018 Jan 1;32(1):79–92. doi: 10.1101/gad.309245.117

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© 2018 Pontes and Groisman; Published by Cold Spring Harbor Laboratory Press

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Figure 5. — Free cytoplasmic Mg²⁺ inhibits Pi starvation response. (A) Fluorescence from wild-type (14028s), mgtA (EG16735), mgtB (EL5), and mgtA mgtB (EG17048) Salmonella harboring pPhoB-GFP. (B) Fluorescence from wild-type Salmonella (14028s) carrying pPstS-GFPc and pUHE-21 (pVector) and from mgtA mgtB mutant (EG17048) harboring pPstS-GFPc and either pUHE-21 (pVector) or pUHE-MgtA (pMgtA) in the presence of the indicated IPTG concentrations. (C) Fluorescence from wild-type Salmonella (14028s) carrying pPstS-GFPc and pVector and from mgtA mgtB mutant Salmonella (EG17048) harboring pPstS-GFPc and either pVector or pUHE-ATPase (pATPase) in the presence of the indicated IPTG concentrations. (D) Fluorescence from mgtA mgtB (EG17048) Salmonella harboring pPhoP-GFP and either pATPase or pVector in the presence of various IPTG concentrations. For all experiments, cells were grown in MOPS liquid medium containing 10 µM MgCl₂ and 500 µM K₂HPO₄. Error bars represent the standard deviations. Graphs are representative of at least three independent experiments with a total of at least six biological replicates. See also Supplemental Figures S2 and S3.