Table 1. Thermotolerant genes identified in this study.
| Classification | Sub-classification | Gene | Function | 30°Ca | 37°Cb | 44°Cc | 45°Cd | 46°Ce | Glcf | Mg2+g | H2O2h |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Energy metabolism (Group A) | Oxidative phosphorylation | atpA | F1 sector of membrane-bound ATP synthase, alpha subunit | - | |||||||
| Oxidative phosphorylation | atpD | F1 sector of membrane-bound ATP synthase, beta subunit | - | + | ++ | S | |||||
| Oxidative phosphorylation | atpG | F1 sector of membrane-bound ATP synthase, gamma subunit | - | ||||||||
| Oxidative phosphorylation | nuoC | NADH:ubiquinone oxidoreductase, chain C, D | - | ||||||||
| Pentose phosphate pathway | gntK | Gluconate kinase 2 | - | + | S | ||||||
| Pentose phosphate pathway | phnN | Ribose 1,5-bisphosphokinase | - | ||||||||
| Ubiquinone/menaquinone biosynthesis | ubiE | Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE | - | ||||||||
| Ubiquinone biosynthesis | ubiH | 2-octaprenyl-6-methoxyphenol hydroxylase | - | - | - | S | |||||
| Ubiquinone biosynthesis | ubiX | Flavin prenyltransferase UbiX | - | ||||||||
| Amino acid metabolism | dapF | Diaminopimelate epimerase | - | - | - | ||||||
| Amino acid metabolism | trpB | Tryptophan synthase, beta chain | - | + | S | ||||||
| Vitamin B6 metabolism | pdxH | Pyridoxin/pyridoxamine 5'-phosphate oxidase | - | - | + | S | |||||
| Outer membrane biosynthesis (Group B) | Lipopolysaccharide biosynthesis | lpxL | Lipid A biosynthesis lauroyltransferase | - | - | ++ | S | ||||
| Secretion system | fimG | Minor component of type I fimbriae | - | ++ | S | ||||||
| DNA repair (Group C) | Putative endonuclease | yraN | Predicted Mrr Cat superfamily | - | ++ | S | |||||
| tRNA modification (Group D) | tRNA modification | yccM | Sulfur relay system, 4Fe-4S membrane protein | - | - | + | |||||
| Others | ABC transporter | cydD | ATP-binding/membrane protein CydD | - | ++ | ++ | S | ||||
| Sodium-proton antiporter | nhaA | Na+:H+ antiporter, NhaA family | - | + | ++ | S | |||||
| Hypothetical protein | yjiY | Putative transporter, Carbon starvation protein CstA superfamily | - | S | |||||||
| Hypothetical protein | ydgH | Uncharacterized deacetylase | - | ++ | S | ||||||
| Hypothetical protein | yaiS | DUF1471 family periplasmic protein | - | S |
a to e“-” means very weak growth at indicated temperatures.
f According to the data in S2 Fig, ratios of growth in the presence of glucose to that in the absence of glucose at 46°C were estimated.
“++” and “+” represent more than 2.0 and 1.5–2.0, respectively.
gAccording to the data in S2 Fig, ratios of growth in the presence of MgCl2 to that in the absence of MgCl2 at 46°C were estimated.
“++” and “+” represent more than 2.0 and 1.5–2.0, respectively.
hAccording to the data in S2 Fig, ratios of growth in the presence of H2O2 to that in the absence of H2O2 at 30°C were estimated.
“S” represents less than 0.5.