Table 2. List of thermotolerant genes in E. coli.
| Classificationa | Gene | Definition | Function |
|---|---|---|---|
| Energy Metabolism (Group A) | aceE | Pyruvate metabolism | Pyruvate dehydrogenase compenent E1 decarboxylase component E1 |
| aceF | Pyruvate metabolism | Pyruvate dehydrogenase, dihydrolipoyltransacetylase compenent E2 | |
| lpd | Pyruvate metabolism | Lipoamide dehydrogenase, E3 component, subunit of three comlexes | |
| lipA | Pyruvate mechanism | Liponate synthase | |
| ackA | Pyruvate metabolism | Acetate kinase A and propionate kinase 2 | |
| rpe | Pentose phosphate pathway | D-ribulose-5-phosphate 3-epimerase | |
| cydB | Respiratory chain | Cytochrome d ubiquinol oxidase subunit II | |
| yhcB | Respiratory chain | Cytochrome d ubiquinol oxidase subunit III | |
| atpAb | Oxidative phosphorylation | F1 sector of membrane-bound ATP synthase, alpha subunit | |
| atpD | Oxidative phosphorylation | F1 sector of membrane-bound ATP synthase, beta subunit | |
| atpG | Oxidative phosphorylation | F1 sector of membrane-bound ATP synthase, gamma subunit | |
| nuoC | Oxidative phosphorylation | NADH:ubiquinone oxidoreductase, chain C, D | |
| gntK | Pentose phosphate pathway | Gluconate kinase 2 | |
| phnN | Pentose phosphate pathway | Ribose 1,5-bisphosphokinase | |
| ubiE | Ubiquinone/menaquinone biosynthesis | Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE | |
| ubiH | Ubiquinone biosynthesis | 2-octaprenyl-6-methoxyphenol hydroxylase | |
| ubiX | Ubiquinone biosynthesis | Flavin prenyltransferase UbiX | |
| dapF | Amino acid metabolism | Diaminopimelate epimerase | |
| trpB | Amino acid metabolism | Tryptophan synthase, beta subunit | |
| pdxH | Vitamin B6 metabolism | Pyridoxine/pyridoxamine 5'-phosphate oxidase | |
| ybhH | Hypothetical protein | Putative isomerase | |
| Outer membrane stabilization (Group B) | gmhB | Lipopolysaccharide biosynthesis | D,D-heptose 1,7-bisphosphate phosphatase |
| lpcA (gmhA) | Lipopolysaccharide biosynthesis | D-sedoheptulose 7-phosphate isomerase | |
| rfaC (waaC) | Lipopolysaccharide biosynthesis | ADP-heptose:LPS heptosyl transferase I | |
| rfaD (waaD) | Lipopolysaccharide biosynthesis | ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding | |
| rfaE (gmhC) | Lipopolysaccharide biosynthesis | Fused heptose7-phosphate kinase and heptose 1-phosphate adenyltransferase | |
| rfaF (waaF) | Lipopolysaccharide biosynthesis | ADP-heptose:LPS heptosyltransferase II | |
| rfaG (waaG) | Lipopolysaccharide biosynthesis | Glucosyltransferase I | |
| lpxL | Lipopolysaccharide biosynthesis | Lipid A biosynthesis lauroyltransferase | |
| ydcL | Peptidoglycan-associated lipoprotein | Predicted lipoprotein | |
| yfgL | Peptidoglycan-associated lipoprotein | Protein assembly complex, lipoprotein component | |
| ynbE | Peptidoglycan-associated lipoprotein | Predicted lipoprotein | |
| nlpI | Peptidoglycan-associated lipoprotein | Conserved protein | |
| ycdO | Peptidoglycan-associated lipoprotein | Conserved protein | |
| pal | Outer membrane integrity | Tol/Pal system, peptidoglycan-associated outer membrane lipoprotein | |
| tolQ | Outer membrane integrity | Tol/Pal system, membrane-spanning protein | |
| tolR | Outer membrane integrity | Tol/Pal system, membrane-spanning protein | |
| yciM | Outer membrane integrity | Conserved hypothetical protein | |
| fimG | Secretion system | Minor component of type 1 fimbriae | |
| DNA repair (Group C) | dnaQ | DNA replication & repair, DSBR | DNA polymerase III subunit, epsilon |
| holC | DNA replication & repair, DSBR | DNA polymerase III subunit, chi | |
| priA | DNA replication & repair, DSBR | Primosome factor n' | |
| ruvA | DNA replication & repair, DSBR | Component of RuvABC resolvasome, endonuclease | |
| ruvC | DNA replication & repair, DSBR | Conserved protein required for cell growth | |
| yraN | Hypothetical protein | Predicted Mrr Cat superfamily | |
| tRNA modification (Group D) | iscS | tRNA modification | Sulfer relay system, cysteine desulfurase |
| yheL (tusB) | tRNA modification | Sulfer relay system, predicted intracellular sulfur oxidation protein | |
| yheM (tusC) | tRNA modification | Sulfer relay system, predicted intracellular sulfur oxidation protein | |
| yheN (tusD) | tRNA modification | Sulfer relay system, predicted intracellular sulfur oxidation protein | |
| yhhP (tusA) | tRNA modification | Sulfer relay system, conserved protein required for cell growth | |
| yccM | tRNA modification | Sulfur relay system, 4Fe-4S membrane protein | |
| miaA | tRNA modification | Delta(2)-isopentenylpyrophosphate tRNA-adenosine transferase | |
| trmU | tRNA modification | tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase | |
| truA | tRNA modification | Pseudouridine synthase A | |
| Chaperone /protease (Group E) | dnaJ | Chaperone system | Chaperone Hsp40, co-chaperone with DnaK |
| dnaK | Chaperone system | Chaperone Hsp70, co-chaperone with DnaJ | |
| degP | Chaperone system | Chaperone/serine endoprotease | |
| rseA | Chaperone regulator | Anti-sigma factor | |
| Translational control (Group F) | rpmJ | Translational control | 50S ribosomal subunit L36, related to secY expression |
| rpsF | Translational control | 30S ribosomal subunit S6, modified with glutamic acid or phosphate | |
| dksA | Translational control | DNA-binding transcriptional regulator or rRNA transcription | |
| smpB | Translational control | Component of trans-translation process | |
| Cell division (Group G) | xerC | Related to cell division | Site-specific tyrosine recombinase for chromosome dimmer resolution |
| dedD | Related to cell division | Membrane-anchored periplasmic protein involved in separation | |
| envC | Related to cell division | Regulator of cell wall hydrolases responsible for cell separation | |
| Transporter (Group H) | zntA | Membrane transport | Zinc/cadmium/mercury/lead-exporting ATPase |
| ybgH | Membrane transport | Predicted proton-dependent oligopeptide transporter, POT family | |
| cydD | Membrane transport | ATP-binding/permease protein CydD | |
| nhaA | Membrane transport | Na+/H+ antiporter, NhaA family | |
| yjiY | Hypothetical protein | Putative transporter, carbon starvation protein CstA superfamily | |
| Others | yaiS | Hypothetical protein | Uncharacterized deacetylase |
| ydgH | Hypothetical protein | DUF1471 family periplasmic protein |
aA new group of transporter (Group H) is added. Transporters were classified in others in previous paper [1].
bThermotolerant genes identified in this study are shown by bold letters.