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. 2018 Jan 3;67(3):400–411. doi: 10.2337/db17-0745

Figure 7.

Figure 7

Reduction of EEF1D and HDAC1/2 expression in 3T3-L1 CAR adipocytes induces inflammation. Adenoviruses expressing interfering short hairpin (sh) RNA for green fluorescent protein (shGFP), EEF1D (shEEF1D), or HDAC1/2 (shHDAC1/2) were used to infect 3T3-L1 CAR adipocytes. A: shEEF1D effectively reduced EEF1D mRNA in 3T3-L1 CAR adipocytes. B: Effect of EEF1D knockdown on expression of TNF, IL6, MCP1, and MCP3 genes. C: shHDAC1/2 effectively reduced HDAC1 and 2 mRNA in 3T3-L1 CAR adipocytes. D–F: Effect of HDAC1/2 knockdown on the expression of TNF, IL6, MCP1, MCP2, MCP3, and Chop genes (D), lipolysis (E), and JNK activation (F). G: Coimmunoprecipitation of SNRK, EEF1D, and HDAC1 from the overexpression study. H: HDAC1 activity in gonadal WAT of SNRKfat−/−, A-Cre and littermate control mice (n = 3 or 4 mice/group). IP, immunoprecipitation; TSA, trichostatin A (a pan HDAC inhibitor); WB, Western blot. *P < 0.05, shGFP vs. shEEF1D or shGFP vs. shHDAC1+2.