Fig 5. Ivermectin impairs Plasmodium vivax development in Anopheles aquasalis and Anopheles darlingi when administered in a double feed 4 days after infection.

An. aquasalis and An. darlingi female mosquitos were fed P. vivax blood samples. Four days after infection, two groups of mosquitos received a second uninfected blood meal with mIVM plasma from control (0 hours) or 4 hours after IVM intake. (A) The proportion of P. vivax infected An. aquasalis and (B) An. darlingi are presented as the mean and standard deviation. (C and D) The intensity of infection is presented as the number of oocysts per midgut (dots) and the black lines represent the median and standard error. Data from 5 independent experiments are presented. Asterisks (*) represent significant difference (P < 0.05) in relation to control.