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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Methods Cell Biol. 2017 Nov 6;143:171–185. doi: 10.1016/bs.mcb.2017.08.010

FIG. 3.

FIG. 3

Dissociation and assembly of collagen IV NC1 hexamer. (A) Dissociation of purified bLBM NC1 hexamers after dialysis in Tris-acetate buffer illustrated by the elution profile form Superdex S200 column. NC1 monomers (16.4 mL peak) were concentrated and used for assembly. Inset shows SDS-PAGE of NC1 hexamer and monomer peaks. (B) Chloride-mediated assembly of NC1 hexamer from purified LBM monomers. Incubation in the presence of NaCl (150 mM, 37°C, 24 h) induces formation of hexamer (peak at 13.2 mL) from NC1 monomers (peak at 16.4 mL).