Fig. 5.

ESAT-6 specific secondary effectors express significantly more KLRG1 than Ag85B-specific CD4 T cells in Mtb memory mice in contrast to H56 memory mice.

Ten weeks into infection of rested C57BL/6 memory mice, the frequency of KLRG1 + cells and the expression levels of KLRG1 within the lung parenchyma (IV –ve population) was assessed after i.v. injection of anti-CD45.2-FITC prior to tissue harvest (unperfused lungs) using I-A^b:Ag85B_280–294 and I-A^b:ESAT-6_4–17 specific tetramer staining. A) Bar chart showing the frequency of Ag85B_280–294 and ESAT-6_4–17 specific CD4 T cells being KLRG1 + within the IV –ve lung population ten weeks into challenge of H56 (white) and Mtb memory (black) mice. Two-way ANOVA with Tukey's multiple comparison test. **P < 0.01. B) Bar chart showing the KLRG1 gMFIs of Ag85B_280–294 and ESAT-6_4-17 specific CD4 T cells within the lung parenchyma (IV –ve) ten weeks into challenge of H56 (white) and Mtb memory (black) mice. Two-way ANOVA with Tukey's multiple comparison test. ***P < 0.001, *P < 0.05. Gating as depicted in Supplementary Fig. 4. The experiment was repeated once with similar results.