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. 2018 Feb 6;115(8):E1809–E1818. doi: 10.1073/pnas.1716582115

Fig. 3.

Fig. 3.

TAC102 does not accumulate upon TAC40 depletion. (A) Cartoon of a trypanosome; boxed region is enlarged on Right. The mitochondrion is depicted in orange, the flagellum in dark gray, the kDNA in blue, and TAC102 in green. After depletion of TAC40 (OM), two scenarios are shown for cells with an enlarged kDNA. In scenario I, the signal for TAC102 does not increase in size or intensity; in scenario II, the TAC102 signal increases. (B) TAC40 RNAi cells uninduced (−Tet, MitoTracker stained, red) and 24 h-induced (+Tet) were mixed and stained with YL1/2 (basal body, yellow), TAC102 (green), and DAPI (DNA, cyan). (C) The integrated density of the YL1/2, TAC102, and DAPI signal was measured with ImageJ from 1K1N uninduced cells (−Tet) and TAC40-depleted cells (+Tet; only cells with enlarged kDNA were quantified) [n(–Tet). = 30, n(+Tet). = 23]. (Scale bar: 3 µm.)