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. 2018 Feb 5;115(8):E1876–E1885. doi: 10.1073/pnas.1718819115

Fig. 3.

Fig. 3.

NR increases in vitro EdU incorporation in NPCs and decreases neuroinflammation. (A) Representative images of EdU (red), Sox2 (green), and DAPI (blue) staining of NPCs treated with vehicle or 1 mM NR for 24 h. (Scale bars, 50 µm.) (B) Quantification of EdU+ cells from images as in A. n = 27 control (−NR) and 26 +NR images in three experiments. (C and D) Representative images of GFAP (red) and IBA1 (green) staining of hippocampus regions (C) and cortex regions (D) from WT, Polβ, AD, and AD/Polβ mice treated with vehicle or NR. (Scale bars: 200 µm in C; 20 µm in C, Inset; 50 µm in D.) (E and F) Quantification of GFAP (E) and IBA1 (F) intensity from sections as in C and D. n = 5 mice per group. Quantifications are presented as mean ± SEM. (G) Indicated cytokine or chemokine levels in mouse plasma detected by multiplex cytokine array. n = 11 (WT + Veh), 10 (Polβ + Veh), 11 (AD + Veh), 12 (AD/Polβ + Veh), 9 (WT + NR), 10 (Polβ + NR), 8 (AD + NR), and 9 (AD/Polβ + NR) mice. Quantifications are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.