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View full-text article in PMC

. 2018 Feb 5;115(8):1865–1870. doi: 10.1073/pnas.1720487115

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Fig. 4. — O₂ affinities of recombinantly expressed Hbs representing wild-type genotypes of Parus humilis and Lophophanes dichrous and the reconstructed genotype of their most recent common ancestor AncParidae. Site-directed mutagenesis experiments revealed that α^AA34T produces a significant affinity-enhancing effect on the ancestral background (AncParidae), as indicated by the reduction in P₅₀, but does not completely recapitulate the evolved increases in Hb–O₂ affinity in Parus humilis or Lophophanes dichrous. Cross-hatched and solid bars show P₅₀ values in the absence (stripped) and presence of anionic cofactors, respectively. Mutational reversions (α^AA34T) on the wild-type backgrounds of Parus humilis and Lophophanes dichrous did not produce a symmetrical, affinity-reducing effect. The asterisk denotes a statistically significant difference (P < 0.05) in the presence of KCl + IHP.