Fig. 1.

Two regions within the coding sequence of GgNAD-ME1 are necessary for preferential gene expression in the bundle sheath (BS). An antisense construct, as well as a deletion series from the 5′ and 3′ ends of GgNAD-ME1 coding sequence were translationally fused to the uidA reporter under the control of the CaMV35S promoter (A). Percentage of cells containing GUS in the BS after microprojectile bombardment of G. gynandra leaves. Bars represent the percentage of stained cells in BS cells, error bars denote the SE. Statistically significant differences with *P values <0.05 and CI = 95% determined by a one-tailed t test (B). GUS in G. gynandra transformants containing uidA fused to 1–240, 1–141, 79–240, and 64–162 base pairs from the translational start site of GgNAD-ME1 (C). (Scale bars, 100 µm.)