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. 2018 Feb 5;115(8):1783–1788. doi: 10.1073/pnas.1721788115

Fig. 2.

Fig. 2.

Trans-interactions between TRAF6 and donor-Ub are critical for Ub transfer. (A) Superimposed gel filtration profiles of TRAF6 wild-type and mutants, showing the preservation of dimeric states in the mutants. The TRAF6 constructs, containing RING and ZF1-3, have a theoretical dimer molecular mass of 37 kDa. mAU, milli absorption units. (B) Promotion of polyubiquitin chain synthesis by wild-type and mutant TRAF6 in the presence of E1, the E2 complex Ubc13/Uev1A, and ubiquitin. (C) Promotion of polyubiquitin chain synthesis by wild-type and mutant TRAF6 at different time points.