Table 1.

Study File Summary. Sixteen bone marrow specimens from different human donors that were determined to have no observable disease were used for this study. In order to be included in the study, the listmode data had to: 1) include CD19, CD34, CD45, CD10, and CD38, 2) have good visible separation between negative and positive staining, and 3) have adequate numbers of events for staging analyses. For the B-cell staging part of the study, the files needed at least 2,500 CD19 dim/+ events and for the progenitor staging, 1,000 CD34+ events. Those files that satisfy the latter requirement have an ‘*’ suffix.

File Name	# of Events	#CD19 dim/+	#Prog. Events	Date Collected	Instrument	Markers Present
						CD19	CD10	CD34	CD38	CD45	CD20	TdT	CD81	CD22	CD44	CD9
B_BM1.fcs*	250000	16811	996	6-May-13	Facs CantoII	x	x	x	x	x				x	x
B_BM2.fcs	100000	2589		23-Apr-14	Facs CantoII	x	x	x	x	x	x	x
B_BM3.fcs	100000	4722		23-Apr-14	Facs CantoII	x	x	x	x	x	x	x
B_BM4.fcs	100000	3430		1-May-14	Facs CantoII	x	x	x	x	x	x	x
B_BM5.fcs	100000	8559		1-May-14	Facs CantoII	x	x	x	x	x	x	x
B_BM6.fcs	100000	5583		1-May-14	Facs CantoII	x	x	x	x	x	x	x
B_BM7.fcs*	250000	22012	15063	11-Jan-12	Facs CantoII	x	x	x	x	x				x	x
B_BM8.fcs*	337097	34849	5450	25-Aug-09	LSRII	x	x	x	x	x	x		x
B_BM9.fcs*	417911	7446	7448	3-Feb-10	LSRII	x	x	x	x	x	x
B_BM10.fcs*	422432	11898	2927	3-Feb-10	LSRII	x	x	x	x	x	x
B_BM11.fcs*	742320	6622	7179	4-Feb-10	LSRII	x	x	x	x	x	x
B_BM12.fcs*	522838	23228	4603	4-Feb-10	LSRII	x	x	x	x	x	x
B_BM13.fcs	171218	32332		10-Feb-10	LSRII	x	x	x	x	x	x					x
B_BM14.fcs*	359633	30549	2476	17-Mar-10	LSRII	x	x	x	x	x	x					x
B_BM15.fcs*	321185	6343	7385	17-Mar-10	LSRII	x	x	x	x	x	x					x
B_BM16.fcs*	415403	10994	4737	20-Mar-10	LSRII	x	x	x	x	x	x					x