Table 2.
B-cell Stage Results. All files were initially analyzed unattended with a B-cell model to obtain critical stage locations for markers CD34, CD45, CD20, CD10, and CD38. The units for the locations are cumulative percentages (see (31) for details on why the cumulative percentages are useful for representing cellular progressions). The recorded results for all sixteen study files are shown above. Markers were deemed to be on the same stage boundary if their correlation coefficients, r, were significant and their t values were not (see bottom of table). Since CD34 (a) versus CD45 L2 (c) (t=9.9, p<0.00001) and CD45 L2 (c) versus CD10 (e) (t=3.5, p<0.004) were found to be significantly different, they were considered to form different stage boundaries.
| B1 | B2 | B3 and B4 | ||||
|---|---|---|---|---|---|---|
| Files |
|
|
|
|
|
|
| B_BM1 | 7.7 | 9.1 | 58.7 | 67.8 | 67.1 | |
| B_BM2 | 27.2 | 29.0 | 96.4 | 95.7 | 95.4 | 94.6 |
| B_BM3 | 14.1 | 21.3 | 71.5 | 70.8 | 78.6 | 77.8 |
| B_BM4 | 19.4 | 26.3 | 65.1 | 65.1 | 74.1 | 71.8 |
| B_BM5 | 5.1 | 7.2 | 79.4 | 76.2 | 92.3 | 92.7 |
| B_BM6 | 3.6 | 3.6 | 18.0 | 20.1 | 24.3 | 23.1 |
| B_BM7 | 7.5 | 8.2 | 73.4 | 47.5 | 79.5 | 59.3 |
| B_BM8 | 9.3 | 9.0 | 71.8 | 71.8 | 81.9 | 89.2 |
| B_BM9 | 48.6 | 50.6 | 98.8 | 100.0 | 96.7 | 95.7 |
| B_BM10 | 6.6 | 8.7 | 37.2 | 37.1 | 41.6 | 40.7 |
| B_BM11 | 11.5 | 12.3 | 83.4 | 82.0 | 83.2 | 82.2 |
| B_BM12 | 4.1 | 4.9 | 24.3 | 24.6 | 26.4 | 26.2 |
| B_BM13 | 2.4 | 6.6 | 95.4 | 97.0 | 97.5 | 95.6 |
| B_BM14 | 5.1 | 5.7 | 37.4 | 37.4 | 40.8 | 39.9 |
| B_BM15 | 41.4 | 66.6 | 81.2 | 80.6 | 78.4 | 81.7 |
| B_BM16 | 33.1 | 35.6 | 92.2 | 93.1 | 91.4 | 91.5 |
| μ | 15.4 | 19.0 | 67.8 | 66.6 | 71.9 | 70.6 |
| σ | 14.5 | 18.4 | 25.9 | 27.0 | 24.8 | 25.3 |
| r | 0.95 | 0.99 | 0.99 | |||
| H0: r=0 | <0.00001 | <0.00001 | <0.00001 | |||
| t | 2.36 | 0.21 | 1.37 | |||
| H0: t=0 | NS | NS | NS | |||
The above statistical analysis suggests the following interpretation of the B-cell stages. The location for the down-regulation of CD34 (a) and the initial up-regulation of CD45 L1 to L2 (b) represent the end of the first stage, B1 (red). The second up-regulation of CD45 L2 to L3 (c) and the up-regulation of CD20 (d) mark the end of the second stage, B2 (green). The down-regulation of CD10 and CD38 (e and f) defined the end of stage B3 (blue) and the beginning of stage B4 (purple, not shown).