Fig. 1.

2′, 7′-dichlorofluorescein (DCF) emissions were lower in myofibers from Ts65Dn mice. A. Fluorescence images of DCF in a myofiber at the start, and after 15 min of incubation at room temperature. Gray values for each myofiber were calculated using Image J. The value at 15 min was expressed relative to the initial value. In this example the relative fluorescence value was 1.43. The brightfield image of the same myofiber is shown on the right. B. Left: Ts65Dn myofibers exhibit significantly reduced basal DCF emissions than myofibers from wild-type animals. N = 3–5 animals/group. 12 total myofibers from Ts65Dn and 27 from wild-type. * p < 0.05. Right: Fifteen minutes of contractile activity did not affect DCF emissions in wild-type or Ts65Dn myofibers compared to basal DCF emission levels. N = 2–3 animals/group. 11 total myofibers from Ts65Dn and 11 from wild-type. Bars are means ± standard error.