Fig. 2.

Protein expression of superoxide dismutase 1 (SOD1) is increased in myofibers from Ts65Dn mice. A. Immunofluorescence labelling of SOD1 from wild-type and Ts65Dn myofibers. Myofibers were labeled for SOD1 and 4′,6-diamidino-2-phenylindole (DAPI) to visualize SOD1 (left; pseudo color red) and nuclei (middle; pseudo color blue). B. Quantification of SOD1 expression from wild-type and Ts65Dn myofibers. Gray values for each myofiber were calculated using Image J. Values were normalized using the mean of the wild-type group. N = 4 animals/group. 14 total myofibers from Ts65Dn and 12 from wild-type. Bars are means ± standard error. * p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article).