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. Author manuscript; available in PMC: 2018 Dec 14.
Published in final edited form as: Nature. 2017 Dec 6;552(7684):248–252. doi: 10.1038/nature25013

Extended Data Figure 5. Association of cholesterol with cadherins and PS1.

Extended Data Figure 5

a, Experimental pipeline for studying the association of proteins with cholesterol in Fig. 3n–o. b, Reverse immunoprecipitation for studying the association of proteins with cholesterol. Note the difference in step 5 and 6 from experimental procedure in a. c–e, Representative Li-cor system images. Proteins clicked with cholesterol were visualized in green; i.e. (c) N-cadherin, (d) VE-cadherin and (e) presenilin1 (PS1-F: full length; PS1-N: N-terminal fragment) were detected on the same membrane and visualized in red. Comparable results were observed in 3 additional experiments.