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. Author manuscript; available in PMC: 2019 Jun 20.
Published in final edited form as: Annu Rev Biochem. 2017 Aug 25;87:783–807. doi: 10.1146/annurev-biochem-062917-011852

Figure 6.

Figure 6

Threshold response of SREBP-2 processing to changes in cholesterol content of purified ER membranes. ER cholesterol content was varied by treating CHO cells for various times with different amounts of cholesterol (a) complexed to MCD or (b) delivered in β-VLDL. Each symbol represents data from a different experiment. The amount of nuclear SREBP-2 at zero time was normalized to 100%. ER cholesterol concentration is expressed as a molar percentage (mol%) of total ER lipids. Best-fit analysis of the data reveals Hill coefficients of (a) 3.7 ± 0.23 and (b) 3.5 ± 0.13. Abbreviations: β-VLDL, β-migrating very-low-density lipoprotein; ER, endoplasmic reticulum; MCD, methyl-β-cyclodextrin; SREBP, sterol regulatory element-binding protein. Reprinted from Reference 4.